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Promega 32p-labelled random-primed probe
32p Labelled Random Primed Probe, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32p-labeled+random-primed+probe/32p+labeled+random+primed+probe/10__1007_slash_s13562___023___00840___6-93-6-8
Average 90 stars, based on 1 article reviews
32p-labelled random-primed probe - by Bioz Stars, 2026-09
90/100 stars

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Random Primed:

Article Title: Dengue virus E glycoprotein production in transgenic rice callus.
Article Snippet: Dengue is a disease caused by dengue virus and represents the most important arthropod-borne viral disease in humans.. Dengue virus enters host cells via binding of envelope glycoprotein (E) to a receptor.. In this study, plant expression vectors containing native and synthetic glycoprotein E genes (sE) modified based on plant-optimized codon usage and fused with an ER retention signal were constructed under control of the rice amylase 3D promoter expression system.

Article Title: Expression of cholera toxin B subunit in transgenic rice endosperm.
Article Snippet: The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1.. The recombinant vector was transformed into rice plants using a biolisticmediated transformation method.. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis.

Article Title: Expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat-labile enterotoxin in tobacco plants.
Article Snippet: The pentameric B subunit of Escherichia coli heat-labile enterotoxin (LTB) can be used as an eYcient mucosal carrier of either immunogenic or tolerogenic T-cell epitopes.. Co-delivery of therapeutic proteins with carrier proteins could increase the eVectiveness of the antigen.. This paper reports the ability of transgenic tobacco plants to express a fusion protein consisting of the synthetic LTB and a synthetic neutralizing epitope of porcine epidemic diarrhea virus (PEDV), causing an enteric disease that is especially severe in piglets.

Article Title: Expression of the synthetic neutralizing epitope gene of porcine epidemic diarrhea virus in tobacco plants without nicotine.
Article Snippet: Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in pigs, leading to death with a high mortality.. In this study, the synthetic neutralizing epitope gene of PEDV, which was optimized based on the coding sequence of tobacco plant genes being modified, was expressed in the no-nicotine tobacco plants.. The synthetic gene was cloned into the plant expression vector under the control of CaMV 35S promoter and transformed by an Agrobacterium-mediated transformation.

Article Title: Expression of the B subunit of E. coli heat-labile enterotoxin in tobacco using a herbicide resistance gene as a selection marker
Article Snippet: The B subunit of Escherichia coli heat-labile enterotoxin (LTB) has been transformed to plants for use as an edible vaccine.. We have developed a simple and reliable Agrobacterium-mediated transformation method to express synthetic LTB gene in N. tabacum using a phosphinothricin acetyltransferase (bar) gene as a selectable marker.. The synthetic LTB gene adapted to the coding sequence of tobacco plants was cloned to a plant expression vector under the control of the ubiquitin promoter and transformed to tobacco by Agrobacterium-mediated transformation.

Article Title: Mass production of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng.
Article Snippet: The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered antigens.. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant.. When transgenic somatic embryos were cultured in 130 L air-lift type bioreactor, they were developed to mature somatic embryos through somatic embryogenesis and contained approximately 0 L t p ©

Article Title: Expression of a Synthetic Cholera Toxin B Subunit in Tobacco Using Ubiquitin Promoter and <I>bar</I> Gene as a Selectable Marker
Article Snippet: The blot was hybridized with a 32P-labeled random-primed (Promega) probe overnight at 60°C in a buffer (pH 7.4) that contained 1 mM EDTA, 250 mM Na2HPO4·7H2O, 1% hydrolyzed casein, and 7% sodium dodecyl sulfate (SDS) in hybridization incubator (Finemould Precision Ind., Korea).

Modification:

Article Title: Dengue virus E glycoprotein production in transgenic rice callus.
Article Snippet: Dengue is a disease caused by dengue virus and represents the most important arthropod-borne viral disease in humans.. Dengue virus enters host cells via binding of envelope glycoprotein (E) to a receptor.. In this study, plant expression vectors containing native and synthetic glycoprotein E genes (sE) modified based on plant-optimized codon usage and fused with an ER retention signal were constructed under control of the rice amylase 3D promoter expression system.

Article Title: Expression of cholera toxin B subunit in transgenic rice endosperm.
Article Snippet: The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1.. The recombinant vector was transformed into rice plants using a biolisticmediated transformation method.. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis.

Article Title: Expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat-labile enterotoxin in tobacco plants.
Article Snippet: The pentameric B subunit of Escherichia coli heat-labile enterotoxin (LTB) can be used as an eYcient mucosal carrier of either immunogenic or tolerogenic T-cell epitopes.. Co-delivery of therapeutic proteins with carrier proteins could increase the eVectiveness of the antigen.. This paper reports the ability of transgenic tobacco plants to express a fusion protein consisting of the synthetic LTB and a synthetic neutralizing epitope of porcine epidemic diarrhea virus (PEDV), causing an enteric disease that is especially severe in piglets.

Article Title: Expression of the synthetic neutralizing epitope gene of porcine epidemic diarrhea virus in tobacco plants without nicotine.
Article Snippet: Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in pigs, leading to death with a high mortality.. In this study, the synthetic neutralizing epitope gene of PEDV, which was optimized based on the coding sequence of tobacco plant genes being modified, was expressed in the no-nicotine tobacco plants.. The synthetic gene was cloned into the plant expression vector under the control of CaMV 35S promoter and transformed by an Agrobacterium-mediated transformation.

Article Title: Expression of the B subunit of E. coli heat-labile enterotoxin in tobacco using a herbicide resistance gene as a selection marker
Article Snippet: The B subunit of Escherichia coli heat-labile enterotoxin (LTB) has been transformed to plants for use as an edible vaccine.. We have developed a simple and reliable Agrobacterium-mediated transformation method to express synthetic LTB gene in N. tabacum using a phosphinothricin acetyltransferase (bar) gene as a selectable marker.. The synthetic LTB gene adapted to the coding sequence of tobacco plants was cloned to a plant expression vector under the control of the ubiquitin promoter and transformed to tobacco by Agrobacterium-mediated transformation.

Article Title: Mass production of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng.
Article Snippet: The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered antigens.. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant.. When transgenic somatic embryos were cultured in 130 L air-lift type bioreactor, they were developed to mature somatic embryos through somatic embryogenesis and contained approximately 0 L t p ©

Article Title: Expression of a Synthetic Cholera Toxin B Subunit in Tobacco Using Ubiquitin Promoter and <I>bar</I> Gene as a Selectable Marker
Article Snippet: The blot was hybridized with a 32P-labeled random-primed (Promega) probe overnight at 60°C in a buffer (pH 7.4) that contained 1 mM EDTA, 250 mM Na2HPO4·7H2O, 1% hydrolyzed casein, and 7% sodium dodecyl sulfate (SDS) in hybridization incubator (Finemould Precision Ind., Korea).

Hybridization:

Article Title: Dengue virus E glycoprotein production in transgenic rice callus.
Article Snippet: Dengue is a disease caused by dengue virus and represents the most important arthropod-borne viral disease in humans.. Dengue virus enters host cells via binding of envelope glycoprotein (E) to a receptor.. In this study, plant expression vectors containing native and synthetic glycoprotein E genes (sE) modified based on plant-optimized codon usage and fused with an ER retention signal were constructed under control of the rice amylase 3D promoter expression system.

Article Title: Expression of cholera toxin B subunit in transgenic rice endosperm.
Article Snippet: The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1.. The recombinant vector was transformed into rice plants using a biolisticmediated transformation method.. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis.

Article Title: Expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat-labile enterotoxin in tobacco plants.
Article Snippet: The pentameric B subunit of Escherichia coli heat-labile enterotoxin (LTB) can be used as an eYcient mucosal carrier of either immunogenic or tolerogenic T-cell epitopes.. Co-delivery of therapeutic proteins with carrier proteins could increase the eVectiveness of the antigen.. This paper reports the ability of transgenic tobacco plants to express a fusion protein consisting of the synthetic LTB and a synthetic neutralizing epitope of porcine epidemic diarrhea virus (PEDV), causing an enteric disease that is especially severe in piglets.

Article Title: Expression of the synthetic neutralizing epitope gene of porcine epidemic diarrhea virus in tobacco plants without nicotine.
Article Snippet: Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in pigs, leading to death with a high mortality.. In this study, the synthetic neutralizing epitope gene of PEDV, which was optimized based on the coding sequence of tobacco plant genes being modified, was expressed in the no-nicotine tobacco plants.. The synthetic gene was cloned into the plant expression vector under the control of CaMV 35S promoter and transformed by an Agrobacterium-mediated transformation.

Article Title: Expression of the B subunit of E. coli heat-labile enterotoxin in tobacco using a herbicide resistance gene as a selection marker
Article Snippet: The B subunit of Escherichia coli heat-labile enterotoxin (LTB) has been transformed to plants for use as an edible vaccine.. We have developed a simple and reliable Agrobacterium-mediated transformation method to express synthetic LTB gene in N. tabacum using a phosphinothricin acetyltransferase (bar) gene as a selectable marker.. The synthetic LTB gene adapted to the coding sequence of tobacco plants was cloned to a plant expression vector under the control of the ubiquitin promoter and transformed to tobacco by Agrobacterium-mediated transformation.

Article Title: Mass production of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng.
Article Snippet: The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered antigens.. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant.. When transgenic somatic embryos were cultured in 130 L air-lift type bioreactor, they were developed to mature somatic embryos through somatic embryogenesis and contained approximately 0 L t p ©

Article Title: Expression of a Synthetic Cholera Toxin B Subunit in Tobacco Using Ubiquitin Promoter and <I>bar</I> Gene as a Selectable Marker
Article Snippet: The blot was hybridized with a 32P-labeled random-primed (Promega) probe overnight at 60°C in a buffer (pH 7.4) that contained 1 mM EDTA, 250 mM Na2HPO4·7H2O, 1% hydrolyzed casein, and 7% sodium dodecyl sulfate (SDS) in hybridization incubator (Finemould Precision Ind., Korea).



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